Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue
نویسندگان
چکیده
منابع مشابه
Toward deciphering proteomes of formalin-fixed paraffin-embedded (FFPE) tissues
Formalin-fixed paraffin-embedded (FFPE) tissue specimens comprise a potentially valuable resource for both prospective and retrospective biomarker discovery. Unlocking the proteomic profile of clinicopathological FFPE tissues is a critically essential step for annotating clinical findings and predicting biomarkers for ultimate disease prognosis and therapeutic follow-up.
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We present a new concept, termed tissue lithography (TL), and its implementation which enables retrospective studies on formalin-fixed paraffin-embedded tissue sections. Tissue lithography uses a microfluidic probe to remove microscale areas of the paraffin layer on formalin-fixed paraffin-embedded biopsy samples. Current practices in sample utilization for research and diagnostics require comp...
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The technique of direct immunofluorescence has been applied to skin biopsy specimens fixed in formalin and embedded in paraffin wax. The results have been compared with those obtained by using snap-frozen biopsy specimens from the same patients. Trypsinisation of the dewaxed material allowed subsequent detection of immunoglobulins, complement, and fibrinogen. When compared to the fluorescence i...
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ژورنال
عنوان ژورنال: BMC Cell Biology
سال: 2008
ISSN: 1471-2121
DOI: 10.1186/1471-2121-9-13